Invasive species transport demo

I recently completed a second AmeriCorps term with the National Park Service, working in the Ocean and Coastal Resources branch. My task was to research ballast water transport of invasive species and report on ways the parks might respond to the issue.

Humans transport organisms all over the world in a variety of ways, from muddy boots to bugs tucked in produce shipments. One prolific vector is ballast water: water that vessels – often very large cargo ships – use to maintain buoyancy in different voyage and cargo conditions. Vessels typically uptake ballast water straight from the ocean and all the little aquatic organisms in it come along for the ride. This includes critters ranging from human pathogens like E. coli to microscopic crab larvae. Ballast water has introduced some very high-profile invasive species to new locales, such as the zebra mussel to the Great Lakes.

Luckily, we’ve developed multiple treatment strategies for reducing the organism load in ballast water, and increasing levels of regulation mean that invasive species transport will continue to decrease over time. (Of course, it’s another one of those environmental issues where you think “if only we started this fifty years ago instead of twenty…”, but I try not to dwell on that too much.)

For the outreach component of my internship, I developed a hands-on activity demonstrating how human activity moves organisms around the world. The activity is simple: a guest pushes a magnetic model boat across the ocean, and it picks up magnetic organisms. When the boat reaches the opposite shore, stronger magnets pull the “organisms” off the boat, representing the introduction of a species to a new location.

Finally, in order to demonstrate that it is possible to prevent species introduction, I included a purple magnetic wand to represent the use of ultraviolet light to disinfect ballast water.

Activity participants can remove organisms with a “UV wand”.

I made three of these activities (in-progress shots below) and they’ve been shipped out for use at Isle Royale National Park and Indiana Dunes National Park. Maybe someday you’ll encounter them out there!

Soil science demo: Hands-on molecules

Back in 2018, I had the opportunity to participate in the Pacific Science Center’s Science Communication Fellowship Program. Not only did I take classes on science communication, I made my very own, hands-on demo of my research topic.

My graduate research focused on how water interacts with soil, addressing the question of why soil sometimes does not absorb water from a molecular standpoint. Most rocks and minerals are readily wettable, so any water resistance must come from the other major component of soil: the organic material from soil microorganisms and decaying plants. I focused on one particular class of molecule, the phospholipid, which forms cell membranes. One end of a phospholipid readily interacts with water; the other does not. The hydrophobic ends of molecules like these were hypothesized to cause soils to repel water. I wanted to find out if and how that might occur.

Demonstrating the whole scale of my project was a big hurdle for designing my demo. I opted to start at the macroscopic and work my way down to the molecular.

The very first time I ran my demo! I would start at the left with a jar of dirt and work across the table to the molecular scale.

Starting off with a jar of dirt, I asked participants to tell me what they saw inside the soil. Typically, participants were quick to find small rocks. I would then ask them if they saw the twigs and pine needles as well. This way, I introduced the concept of soil’s two major components: inorganic (rock and mineral) and organic (plant and animal matter).

Next, I had a box full of plush mineral grains, single-cell organisms, and plant detritus. I asked participants if they could find all the soil components they saw in the real dirt in the box. I included a big drop of water to spark conversation about how water might move through soil. Most participants guessed quickly that the purple blobs were bacteria and other microorganisms, which let me segue into talking about cell membranes and the molecules that make them up.

Magnified “dirt”. Green = plant detritus, purple = microorganisms, brown = sand and clay, blue = water.

Lipids can self-aggregate in a number of ways depending on their surroundings; they have polar ends that interact with water, and long carbon tails that do not interact with water. To demonstrate the behavior of lipids, I made model lipids with magnetic “heads” and velcro “tails.” I also made magnetic water droplets to demonstrate how the different ends of lipids behave. Once visitors had played with the molecules and figured out how they interacted with each other and water, I would ask them to form their own hypothesis for how lipids could make soil hydrophobic. (See photo gallery below. Purple = lipid head groups, yellow = lipid tails.)

Having now done this demo several times, I’ve seen that the magnetic/velcro molecules get the best reception from visitors. Once in-person interaction resumes, I plan to revamp the activity to mostly focus on the molecular scale. I will make magnetic “mineral grains” so that participants can build the lipid structures that have been hypothesized to form on mineral surfaces. As a bonus, since I’ve now finished my Ph.D. project, I can tell them if their results match mine!

Visualizing D&D

Since I’m not currently writing code for my research, I’m keeping myself entertained by plotting Dungeons and Dragons stats. My first plot here is a visualization of how health varies by class in 5th edition – trying to put in perspective how relatively “squishy” a spellcaster is versus everyone else.

Turns out a lot of classes use a d8.

Note that all these lines assume a constitution of 10 (CON+0). Someday I will have a plot of what health looks like if every time there’s a chance to add points to stats, the player chooses to maximize constitution.

So what’s up with scotch tape?

I do a lot of Atomic Force Microscopy (AFM) for my graduate research. AFM uses a very, very small stick to determine the topography of a sample. Think of a topographic map of the mountains, but the scale bar is on the order of a hundred nanometers, rather than a few miles.

Because AFM can detect picometer-scale changes in topography, it’s important to have a flat, clean surface to put your sample material on. I’m talking… as close to atomically flat as we can get.

Luckily, nature makes a really cool substance that does this: mica! You can think of mica as like a stack of slightly-sticky paper. When you peel off the top sheet of paper, you’re left with a pristine surface. Now let’s shrink that down so that the sheet of paper is only a couple nanometers tall and made of aluminum and silicon oxide. Peeling mica nicely by hand presents a challenge – how do you get a grip on something that small?

Tape!

Here I am holding a piece of mica that’s glued to a round magnet. I’ve just pressed a piece of regular tape to the mica surface.

Pressing a piece of scotch tape against the mica surface and then ripping it off can get you that perfect surface, as a couple layers of mica come off with the tape. This is similar to how graphene was first made – much like mica, graphite has a sheet-like structure that can be peeled apart. In fact, for some AFM samples, graphite is a really great substrate.

When I rip the tape off, I’m left with that shiny circle stuck to the tape – that’s a few layers of mica!

That’s the explanation for my blog header: Scotch tape is an indispensable research tool for me! Plus, I really enjoy putting in fancy purchase orders with thousands of dollars of highly specialized AFM equipment… and ten dollars of scotch tape tagged on at the end.

Seasonal contrast

Just another fun comparison of a hike at different points in the year.

Granite Lake in October.
November.
February. Not quite the same angle due to a bunch of leaning trees blocking my usual spot.

Backpacking: Deer Pee Edition

Vacation/relaxation, at this point, means “get outside of cell service range and as far from a road as possible” for me. Which is why, as soon as classes ended, I skedaddled out to Olympic National Park for two nights.

Lots of new experiences were had. For starters, I’ve never camped on a beach before. After the initial mild panic about if I’d identified the high tide line incorrectly and might get swept off into the ocean, tent and all, it was pretty darn cool. Other than a couple of very adorable, very small mice, the only wildlife sighting here was maybe a pod of otters out at sea; alas, I didn’t have binoculars to confirm. Regardless, the scenery was excellent.

Somehow I’d never actually made it out to the coast til now, in spite of living in a state with an actual ocean coastline for almost four entire years.

The next night I hiked out into the rainforest. There are some huge trees there! I hike in a lot of forest that’s recovering from logging, and had therefore forgotten what old growth looks like.

Look at that beast of a tree.

This is also where the deer pee comes in. I was filtering water and heard a noise a little ways off. I look over and hey, cool, a doe!

It was surprisingly ok with getting this close to me. I hope people haven’t been feeding it.

After getting a drink, the doe kept crossing the river. It paused halfway across to pee directly into the water. While I’m glad I was upstream of this particular incident, I can only assume that I’ve drank a lot of deer piss in my time outside. Alas.

Red/yellow columbine. Heck yeah.

In addition to the animal life, there was also an abundance of cool plants and fungi. The main floral highlight for me was the large number of red columbines along the river. I’ve only ever seen blue ones before, so this was fun. As with most hikes I’ve been on, there were many excellent mushrooms, but this time I finally bought a mushroom field guide (which I am referring to as my mushroom bible, since it’s got nice faux leather covers, thin paper and tiny typeface).

These particular mushrooms look like turkey-tail, or another thin polypore.

Same trail, different seasons

One thing I very much enjoy is revisiting trails at different points of the year. Lots of cool comparisons to be made.

March
April
June

Same sign, same dog. The amount of snow is also directly proportional to how tired we were when we got to the sign, because it turns out that walking/snowshoeing through snow is much harder than walking on dirt.

Season: forest fire smoke
Season: heck yeah
Season: I almost slipped on ice and died on the way up here

The view from the summit of the mountain also changes considerably with the seasons. One of my favorite parts of the pictures above is that they’re all taken at roughly 1:30pm, but the lighting is radically different due to cloud cover/smoke/time of the year. That said, if you’re looking for mountain views, the there’s really only three scenarios around here: crisp and clear, raining, or smoke/fog. It saddens me immensely that smoke is projected to become the norm for much of the summer. That forecast inevitably leads me down a rabbit hole of sadness about climate change and all the people/companies that could’ve made better choices, so I’m gonna derail that train of thought for now. I’m supposed to be on vacation.

All that said, I’m curious to see if the March snowfall in the first picture is typical; this year Western Washington got its heaviest snowfall in nearly a century. I’ll report back next spring…

Backpacking: Cold water and… more cool fungi

I took advantage of not having class on Monday to go backpacking for a night. Technically, I could do this on two-day weekends too, but sometimes the thought of having to fit in doing laundry and cleaning the bathroom in addition to unwinding makes me very anxious.

Look at all that water!

Since it’d be two days of hiking, I felt like it was actually worth driving nearly two hours away (including nine miles up a pothole-riddled dirt road. And here my round little commuter car thought it was signing up for an urban lifestyle). The payoff was almost immediate: within a mile I was standing on a bridge over a wide, roaring river. This river started as the outlet to the first of four lakes I visited.

Pictured: Tasty glacial melt.

The lake inlet, on the other hand, did not have a bridge, and on account of my stubby legs/hefty backpack I wound up wading across it barefoot. The water was cold enough to hurt. When my feet went numb, it was a relief. I got my revenge by refilling my water bottles there. Ha, take that, river! A whole two liters less water for you!

Heck yeah.

At this point, the sun emerged, and it took way longer to walk by the next two lakes on account of how often I was getting my socks knocked off by the scenery. (Not literally. The river crossing meant that those cozy wool socks were staying put.)

Heck yeah!

The last lake was at the bottom of a nice long descent. After snagging a campsite, I ate a concerning number of energy bars and salty dehydrated camping food, then set an alarm for 3am, because it ain’t backpacking without at least checking to see if the stars are out. Luckily, the weather forecast was completely wrong, and the sky was clear enough that I could even see satellites crossing the sky. I hope we figure out some way around light pollution in urban areas because that was unbelievably soothing.

And, of course, there were the fungi, both large and small. I found trees that were more mushroom than tree and also wee little orange mushrooms hiding in crevasses barely an inch or two across. Still don’t know anything about them (adding that to my list of things I want to do).

Adventures in publishing science

I’m in the process of publishing my first paper. It’s been interesting.

One thing that’s continually bothered me when reading papers is how general the methods section is. If I’m trying to do the same procedure, not having the details is very unhelpful. Thinking about this while writing mine, I included enough detail to replicate the experiment… only for the editor to ask me to reduce the length of the paper by 30%, and given that the results and conclusions are critical parts of the story, it was the methods section that got cut. So, I’m sorry to all the authors I internally grumbled at while digging around for experimental details. And I’m very disheartened to find that my frustration is actually with the editors.

I also have to wonder why there’s a discrepancy between what the editors want and what I want out of my paper. I want the people in the field, doing similar projects, to know exactly how I did my science- especially if they’re going to try replicating my experiments. But the editor is going to be more concerned with getting more people reading the journal. We have different audiences in mind.

These differences would be easier to deal with if we weren’t in an epoch of separate Supplements which don’t download with the paper. Papers are getting shorter, supplements are getting longer, and I’m tired of having to go back to the paper’s webpage to look for a link to the SI. I’d rather have longer methods sections that I can simply skip over if I’m not interested in how the experiments were done, but would settle for having the supplements attached to the paper.

At any rate, my paper is currently under review, which is good because I need publications to graduate and get a job. And there’s a lot of satisfaction in finally getting several years of work out the door!

Bushwhacking: Nope weed and cool fungi

I hiked an old logging road today, which started out pretty clear and then became overgrown, and then turned into a trail-less bushwhack. I cleared a lot of spiderwebs with my face and will therefore not be revisiting this trail during the annual late summer Spiderpocalypse.

Outside! Yeah!

I also encountered some really neat fungi. As someone who grew up in a desert, the prevalence of mushrooms in the Pacific Northwest has yet to get old.

Heck yeah, forbidden gummy candy. (I have no idea if this is an edible mushroom.)
Probably one of the coolest shelf mushrooms I’ve seen. Looks like it changed orientations after the tree fell.
Adorable lil mushrooms. I don’t see these too frequently, so it’s always a treat.

Unfortunately for my skin, this hike also included a large quantity of Devil’s Club, which I am simply going to call “Nope Weed.”

See? Lots of Nope.
Check out all that Nope.

This route also crossed some wonderful clear creeks. That’s another Pacific Northwest feature which still endlessly delights me.

Not shown: the author’s damp socks.

I’m happily sore and will enjoy not walking 12 miles tomorrow.